Czasopismo | ELEKTRONIKA - KONSTRUKCJE, TECHNOLOGIE, ZASTOSOWANIA | Rocznik 2010 - zeszyt 11

Image sensor - based fluorescence detection for lab-on-a-chip

RAFAŁ WALCZAK

nr katalogowy: 55997

Fluorescence detection is commonly used in analytical chemistry as an optical sensing method. Well known fluorescencebased methodologies developed for “large-scale" analytical devices are transferred to micro-world of microfluidical chips dedicated for chemical and biochemical analyzes. These chips are commonly called lab-on-a-chips (LOC). LOC utilizing fluorescence detection are successfully applied at laboratory level in many fields of life-sciences - for example in separation of biological material by electrophoresis [1] or amplification of genetic material by polymerase chain reaction (PCR) with realtime analyze of amplification kinetics (real-time PCR) [2]. However, the detection issues arise while analytical systems are miniaturized. Reduction of analyzed volume means reduction in detection volume, decrease of analyte available for detection and making it more difficult to detect [3]. Therefore, only high sensitivity and expensive photodetectors co-working with sophisticated electronics are applied to ensure proper optical signal detection from nano- and picoliter volumes of analyte being detected in LOC. On the other hand, it is obvious that “conventional" laboratory fluorescence detection systems are too bulky and expensive to be applied in portable devices utilizing LOC. Therefore, new methodologies and technical solutions must be used to fulfill requirements of LOC - based portable devices. In this paper a brief review of the state-of-the-art of systems for fluorescence detection in LOC is presented. Following, description of a novel image sensor-based fluorescence detection unit co-working with LOC is presented. Finally, example of miniaturized device utilizing described here sensing system co-working with LOC is shown. Fluorescence detection in LOC Scheme of the typical optical path for fluorescence induction and detection from LOC is shown on Figure 1. Commonly used integrated apparatus for fluoresce[...]

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